Procalcitonin (PCT)

PCT Antibody

Name	Anti-Human Procalcitonin (PCT) antibody
Catalog #	R58w3	R150e6	R151h2
Platforms Pairs	Chemiluminescence immunoassay (CLIA)	Immunochromatography assay (ICA)	Turbidimetric Immunoassay (TIA)
Platforms Pairs	R58w3 (Capture) -R150e6 (Detection) R151h2 (Capture) -R150e6 (Detection)	R150e6 (Capture) - R151h2 (Detection)	R150e6 R151h2
Description	Mouse monoclonal antibody, cultured in vitro
Buffer	1xPBS
Purity	Purity>98%, purified by Protein A/G chromatography
Storage	Aliquot and store at -20°C or lower. Avoid freeze / thaw cycles.

PCT Antibody Conjugates

PCT Antibody Conjugated Magnetic Beads

Name	Catalog #
PCT antibody conjugated magnetic beads	B123l1
	B124l1
	B125l1

Labeled PCT Antibody

Name	Catalog #
Biotin labeled PCT antibody	L126l1
Acridinium ester (AE) labeled PCT antibody	L120l1
Alkaline phosphatase (ALP) labeled PCT antibody	L122l1

PCT Antigen

Name	Procalcitonin (PCT) antigen
Description	Recombinant, C-terminal His-tagged, in vitro expressed from E. coli
Applications	Calibrator and quality control product
Catalog #	P1103
Purity	>90%, analyzed by R250-stained SDS-PAGE
Buffer	1 x PBS，pH 7.4
Storage	Aliquot and store at -80°C. Avoid freeze / thaw cycles.
SDS-PAGE	Predicted MW around 13.7 kDa

Product Information

Alkaline phosphatase-labeled Chemiluminescence (ALP-CLIA) platform

Clinical comparative analysis
R58w3-R150e6 matched antibody pair was verified in Roche^TM serum assigned samples (113 cases, concentration: 0.018-46.150ng/mL, clinical coefficient of determination R² > 0.99).

Sample No.	Concentration of Samples （ng/mL）	Concentration of Detection （ng/mL）
1	0.018	0.015
2	0.033	0.028
3	0.016	0.018
...	...	...
112	27.584	28.145
113	46.150	46.200

Fig.1 Correlation of PCT clinical samples on ALP-CLIA platform

Table.1 Data of clinical comparative analysis

Time-resolved immunofluorescence (TRFIA) platform

Correlation in clinical samples
R150e6-R151h2 matched antibody pair was verified in Roche^TM serum assigned samples (30 cases, concentration: 0.16-68.64ng/mL, clinical coefficient of determination R² > 0.96).

Sample No.	Concentration of Samples （ng/mL）	Concentration of Detection （ng/mL）
1	0.16	0.15
2	0.29	0.30
3	0.43	0.42
...	...	...
29	56.28	62.51
30	68.64	58.72

Fig.2 Coincidence rate of PCT clinical samples on TRFIA platform

Table.2 Data of clinical comparative analysis

Diagnostic Significance of Procalcitonin (PCT) Antigen

Inflammatory indicator -PCT

Procalcitonin (PCT) is the precursor peptide of calcitonin (CT). After translated by CT-mRNA, PCT is digested enzymatically into the final product mature CT with 32 amino acids. The content of PCT is extremely low in serum of normal human. After being infected by microbes, the CALC-I gene is induced to express and promote the secretion of PCT until reaching its peak after 8 hours. Currently, PCT has been recognized as a bioindicator for systemic inflammatory response syndrome and has been widely applied in guiding treatments, reducing the use of antibiotics, diagnosing sepsis, and improving long-term outcomes.

Guidance on antibiotics uses

World Health Organization revealed that only 20 out of every 100 cases of respiratory infections require antibiotic treatments. However, conventional diagnostic methods using bacterial culturing for infection often results in delays. Therefore, an indicator that specifically targeting bacterial infections would be most helpful. Research has discovered that PCT can serve as an important indicator differentiating infectious and non-infectious inflammation, as well as distinguishing between bacterial and viral infections. Moreover, the dynamic levels of PCT have important prognostic significance.

Diagnosis of sepsis

Systemic inflammatory response syndrome (SIRS) is the most common manifestation of patients with severe disease diagnosed. When SIRS exists and infection is confirmed or suspected, the term sepsis is used. In severe cases of infection, most pro-inflammatory cytokines (such as TNF-α, IL-1b or IL-6) only increase briefly or intermittently. By contrast, the level of PCT increases several times to several thousand times in sepsis, and such increase is usually associated with the severity of the condition and subsequent mortality rate at admission. Various studies and reviews have indicated that PCT has higher diagnostic accuracy compared to other parameters for diagnosing sepsis.

Reference Value of PCT

<0.05 ng/ml	Normal
<0.5ng/ml	No or slight systemic inflammation
0.5-2ng/ml	Medium level of systemic inflammation
2-10ng/ml	Suspected sepsis
≥10ng/ml	Critical bacterial sepsis

References

[1] Becker KL, Nylén E S, WhiteJ C, et al. Clinical review 167: Procalcitonin and the calcitonin gene family of peptides in inflammation, infection, and sepsis: a joumey from calcitonin back to its precursors.[J]. Journal of Clinical Endocrinology & Metabolism, 2004, 89(4): 1512.
[2] Weglohner W R D, StruckJ S c, Morgenthaler N, et al. Isolation and characterization of serum procalcitonin from patients with sepsis.[J]. Peptides, 2001, 22(12): 2099-103.
[3] Müller B, White J C, NylénE s, et al. Ubiquitous expression of the calcitonin-i gene in multiple tissues in response to sepsis[J]. Journal of Clinical Endocrinology & Metabolism, 2001, 86(1): 396.
[4] Levin A S. Overcoming Antimicrobial Resistance[J]. Brazilian Journal of Infectious Diseases, 2008, 12: 10-21.